Reverse Transcriptase

Product Information

Product Name	Product Code	Size
Reverse Transcriptase	ZS0001ES20	20KU
	ZS0001ES50	50KU
	ZS0001ES100	100KU

Product Description

MMLV Reverse Transcriptase is derived from Moloney Murine Leukemia Virus (MMLV). This reverse transcriptase is genetically engineered via site-directed mutagenesis. It can efficiently synthesize first-strand cDNA up to 15 kb in length. The mutations improve its thermal stability, delivering high reverse transcription efficiency for GC-rich fragments or RNA with complex secondary structures. The synthesized first-strand cDNA is widely applicable for second-strand synthesis, hybridization, PCR amplification, and Real-Time PCR.

Product Properties

Source	E.coli
Molecular Weight	79.0 kDa
Isoelectric Point	8.82
Purity	≥95%
Enzyme Activity	250–300 U/μL
Specific Activity	≥1.5×10⁶ U/mg protein
Storage Buffer	20 mM Tris-HCl pH 7.5 @ 25℃, 100 mM NaCl, 0.2 mM EDTA, 1 mM DTT, 0.1 mg/mL BSA, 0.01% Tween 20, 50% Glycerol.
Unit Definition	One unit is defined as the amount of enzyme required to catalyze the incorporation of 1 nmol deoxyribonucleotide into acid-precipitable material within 10 minutes at 37 ℃.

Applications:

• RT-PCR

• First-strand cDNA synthesis

• cDNA probe preparation

• Construction of cDNA libraries with a high proportion of full-length cDNAs

Shipping and Storage

Shipped on dry ice. Store at -15 ℃ ~ -25 ℃; valid for 2 years. Thaw 5×RT Buffer 3.0 and 0.1 M DTT at room temperature before use, and immediately return to frozen storage after use.

Heat inactivation: 5 min at 85 ℃.

Product Features:
1. High purity
Purity of reverse transcriptase >95% as determined by SDS-PAGE.

2. Wide temperature tolerance
RNA templates were reverse-transcribed at different temperatures, and the resulting cDNA was amplified by qPCR. A no-reverse-transcriptase negative control was included. The results show that this reverse transcriptase maintains excellent reverse transcription performance at 42 ℃, 50 ℃ and 56 ℃.