surzyme Reverse Transcriptase
Product Information
| Product Name | Product Code | Size |
| Reverse Transcriptase | ZS0001ES20 | 20KU |
| ZS0001ES50 | 50KU | |
| ZS0001ES100 | 100KU |
Product Description
MMLV Reverse Transcriptase is derived from Moloney Murine Leukemia Virus (MMLV). This enzyme is genetically modified via site-directed mutagenesis. It efficiently synthesizes first-strand cDNA up to 15 kb. Mutations improve its thermal stability and deliver excellent reverse transcription efficiency for GC-rich fragments or RNA with complex secondary structures. The synthesized first-strand cDNA is widely applicable for second-strand synthesis, hybridization, PCR amplification and Real-Time PCR.
Product Properties
| Source | E.coli |
| Molecular Weight | 79.0 kDa |
| Isoelectric Point | 8.82 |
| Purity | ≥95% |
| Enzyme Activity | 250–300 U/μL |
| Specific Activity | ≥1.5×106 U/mg protein |
| Storage Buffer | 20 mM Tris-HCl pH 7.5 @ 25℃, 100 mM NaCl, 0.2 mM EDTA, 1 mM DTT, 0.1 mg/mL BSA, 0.01% Tween 20, 50% Glycerol. |
| Unit Definition | One unit is defined as the amount of enzyme required to catalyze the incorporation of 1 nmol deoxyribonucleotides into acid-precipitable material within 10 minutes at 37 ℃. |
Applications:
• RT-PCR
• First-strand cDNA synthesis
• cDNA probe preparation
• Construction of cDNA libraries with high full-length cDNA ratio
Shipping and Storage
Shipped on dry ice. Store at -15℃ ~ -25℃; valid for 2 years. Thaw 5×RT Buffer 3.0 and 0.1 M DTT at room temperature before use, and freeze immediately after use.
Heat Inactivation: Inactivate at 85℃ for 5 minutes.
Product Features:
1. High purity
Purity of reverse transcriptase >95% verified by SDS-PAGE.
2. Wide temperature tolerance
Reverse transcription of RNA templates is performed at different temperatures, followed by qPCR amplification of generated cDNA, with a negative control set without reverse transcriptase. Results demonstrate robust reverse transcription performance at 42℃, 50℃ and 56℃.
Notes
This product is for research use only. Not for pharmaceutical, clinical therapeutic, food or cosmetic applications.