Zhongshuo Bio's Pichia pastoris cell-free protein synthesis kit uses the AOX1 promoter to drive transcription of the target gene in vitro, completes mRNA synthesis through the yeast's endogenous RNA polymerase II, and relies on ribosomes and translation factors retained in the lysate to achieve protein expression. The system requires no addition of exogenous RNA polymerase and can achieve efficient protein synthesis within 3 hours; it has been successfully used for the in vitro synthesis of green fluorescent protein, food enzymes, and membrane proteins.

1 Core Product Advantages

✅ No live cells needed

Add template DNA or linear PCR products directly to the reaction system to rapidly initiate expression.

✅ No exogenous RNA polymerase required

The system contains a complete set of "components" required for DNA transcription and translation: ribosomes, translation factors, aminoacyl-tRNA synthetases, total tRNA, energy regeneration components, etc.

✅ Express quickly in 3 hours

Standard reaction: incubate at 24°C for 3 h, after which fluorescence detection, SDS-PAGE, Western Blot, and other downstream analyses can be performed.

✅ Both soluble proteins & membrane proteins can be tried

Suitable for a variety of protein expression needs, with successful cases including membrane proteins, food enzymes, GFP, and more.

2 Product Usage Scenarios

Membrane Protein In Vitro Expression & Functional Studies

High-Throughput Screening of Food/Industrial Enzymes

High-toxicity protein expression✨

Rapid protein mutant validation⏱️

Early-stage sample preparation for structural biology

Dedicated alternative solutions for difficult-to-express proteins

3 Storage and Usage Specifications

Storage conditions: Store all components at -80°C; shelf life is 6 months under recommended conditions.

Thawing requirements: Thaw all components on ice, and complete all experimental procedures within 1 hour after thawing.

Critical prohibition: Do not subject to repeated freeze-thaw cycles, as this will directly impair component activity and reduce protein expression efficiency.

Aliquoting recommendation: After the initial thaw, aliquot as needed into single-use amounts, flash-freeze in liquid nitrogen, and return to -80°C for storage to preserve activity long-term

4 Contact Us

Zhongshuo Bio Pichia pastoris Cell-Free Protein Synthesis Kit breaks through the technical limitations of traditional in vivo protein expression. With its core advantages of ultra-fast speed, convenience, broad applicability, and stability, it is perfectly suited to a variety of scenarios, including research-grade protein expression, enzyme preparation R&D, and membrane protein mechanism research. No tedious bacterial culture procedures are required, and efficient results are obtained in 3 hours, greatly lowering the experimental threshold and saving research time. It is a preferred tool for in vitro protein expression!

For product details, experimental technical support, or sample trials, please feel free to leave a message for inquiries or contact us directly by phone at 17717895428!