surzyme Reverse Transcriptase
Product Information
| Product Name | Product Code | Size |
| Reverse Transcriptase | ZS0001ES20 | 20KU |
| ZS0001ES50 | 50KU | |
| ZS0001ES100 | 100KU |
Product Description
MMLV Reverse Transcriptase is derived from Moloney Murine Leukemia Virus (MMLV). This reverse transcriptase is genetically engineered via site-directed mutagenesis. It can efficiently synthesize first-strand cDNA up to 15 kb in length. The mutations improve its thermal stability, delivering high reverse transcription efficiency for GC-rich fragments or RNA with complex secondary structures. The synthesized first-strand cDNA is widely applicable for second-strand synthesis, hybridization, PCR amplification, and Real-Time PCR.
Product Properties
| Source | E.coli |
| Molecular Weight | 79.0 kDa |
| Isoelectric Point | 8.82 |
| Purity | ≥95% |
| Enzyme Activity | 250–300 U/μL |
| Specific Activity | ≥1.5×106 U/mg protein |
| Storage Buffer | 20 mM Tris-HCl pH 7.5 @ 25℃, 100 mM NaCl, 0.2 mM EDTA, 1 mM DTT, 0.1 mg/mL BSA, 0.01% Tween 20, 50% Glycerol. |
| Unit Definition | One unit is defined as the amount of enzyme required to catalyze the incorporation of 1 nmol deoxyribonucleotide into acid-precipitable material within 10 minutes at 37 ℃. |
Applications:
• RT-PCR
• First-strand cDNA synthesis
• cDNA probe preparation
• Construction of cDNA libraries with a high proportion of full-length cDNAs
Shipping and Storage
Shipped on dry ice. Store at -15 ℃ ~ -25 ℃; valid for 2 years. Thaw 5×RT Buffer 3.0 and 0.1 M DTT at room temperature before use, and immediately return to frozen storage after use.
Heat inactivation: 5 min at 85 ℃.
Product Features:
1. High purity
Purity of reverse transcriptase >95% as determined by SDS-PAGE.
2. Wide temperature tolerance
RNA templates were reverse-transcribed at different temperatures, and the resulting cDNA was amplified by qPCR. A no-reverse-transcriptase negative control was included. The results show that this reverse transcriptase maintains excellent reverse transcription performance at 42 ℃, 50 ℃ and 56 ℃.