Cod UNG (Thermolabile Uracil-DNA Glycosylase from Atlantic Cod)

   Thermolabile uracil-DNA glycosylase originates from Atlantic Cod. It efficiently hydrolyzes uracil residues in single-stranded or double-stranded DNA to form abasic sites. Cod UNG shows no activity against RNA and is mainly used for preventing PCR amplicon carryover contamination.

Overview:

   Cod UNG is a recombinant uracil-DNA glycosylase from Atlantic Cod, a thermolabile enzyme for molecular biology research and diagnostic applications. Its key characteristic is high activity at low temperatures, followed by rapid, complete and irreversible inactivation upon heat treatment. Compared with other commercially available UNG enzymes, Cod UNG cannot be reactivated after heat inactivation. It eliminates residual PCR contaminants without degrading newly-synthesized amplicons, making downstream analysis of PCR products feasible.

Cod UNG cleaves the N-glycosidic bond between deoxyribose and uracil bases in DNA and generates abasic sites, while it has no activity toward uracil in RNA.

Applications:

  The thermolabile UNG from Atlantic Cod is fully and irreversibly inactivated after incubation at 55℃ for 5 min. Therefore, Cod UNG can be added to the PCR master mix before amplification. Incubation at 25℃ for 10 min removes residual PCR amplicons. Subsequent denaturation step at 94℃ during PCR cycles inactivates the enzyme and will not interfere with the generation of new dU-containing PCR products.

• Removal of uracil bases from single‑stranded or double‑stranded DNA

• Elimination of PCR carryover contamination

Features:

• Thermolabile
• Fully irreversible heat inactivation
• Excellent performance in qRT‑PCR and qPCR
• No degradation of PCR amplicons after amplification
• High purity, free of nuclease contamination

Product Specifications:

Storage Buffer:

The enzyme is stored in 20 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1% Triton X-100, 50% Glycerol, pH 7.5 @ 25℃.

Reaction Buffer:

Cod UNG is compatible with most PCR polymerase buffers; its activity will be inhibited when ionic strength >100 mM.

Unit Definition:

One unit is defined as the amount of enzyme required to release 60 pmol uracil from uracil-containing double-stranded DNA within 1 min at 37℃ under standard reaction conditions.

Source:

Expressed and purified in E.coli; recombinant protein produced by expressing Atlantic Cod uracil-DNA glycosylase gene in E.coli via recombinant DNA technology.

Storage Conditions:

Store at -20℃; avoid repeated freeze-thaw cycles.

Heat Inactivation:

Inactivate at 55℃ for 5 minutes.

Precautions:

The activity of Cod UNG is inhibited under high ionic strength conditions.